Primers Available
Organism:Squalidus wolterstorffi mitochondrion
Repeat:(TA)8
Start:16475 End:16490
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCCTTGTCAAACCCCAAAAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTTGTGGAGGAATTGCATGA | |||||||||||||
| Tm_F_1 | 59.807 | Size_F_1 | 20 | Tm_R_1 | 60.049 | Size_R_1 | 20 | PRODUCT Size | 136 bp | Primer Start | 16395 | Primer End | 16530 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAACCCCAAAACCAAGGAAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTTGTGGAGGAATTGCATGA | |||||||||||||
| Tm_F_2 | 60.193 | Size_F_2 | 20 | Tm_R_2 | 60.049 | Size_R_2 | 20 | PRODUCT Size | 128 bp | Primer Start | 16403 | Primer End | 16530 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATCCTTGTCAAACCCCAAAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTTGTGGAGGAATTGCATGA | |||||||||||||
| Tm_F_3 | 59.264 | Size_F_3 | 20 | Tm_R_3 | 60.049 | Size_R_3 | 20 | PRODUCT Size | 137 bp | Primer Start | 16394 | Primer End | 16530 |