Primers Available
Organism:Squalidus wolterstorffi mitochondrion
Repeat:(CTA)4
Start:14747 End:14758
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATTTCGACCGCATTCTCATC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CATTTGTCCTCAGGGAAGGA | |||||||||||||
| Tm_F_1 | 60.043 | Size_F_1 | 20 | Tm_R_1 | 60.042 | Size_R_1 | 20 | PRODUCT Size | 240 bp | Primer Start | 14566 | Primer End | 14805 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGCCCGAGGCCTATACTATG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CATTTGTCCTCAGGGAAGGA | |||||||||||||
| Tm_F_2 | 60.074 | Size_F_2 | 20 | Tm_R_2 | 60.042 | Size_R_2 | 20 | PRODUCT Size | 121 bp | Primer Start | 14685 | Primer End | 14805 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGCCCGAGGCCTATACTATG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGAATGCGAAAAATCGTGTT | |||||||||||||
| Tm_F_3 | 60.074 | Size_F_3 | 20 | Tm_R_3 | 59.945 | Size_R_3 | 20 | PRODUCT Size | 251 bp | Primer Start | 14685 | Primer End | 14935 |