Primers Available
Organism:Squalidus wolterstorffi mitochondrion
Repeat:(AAC)4
Start:4775 End:4786
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACATCTGCAGCCTTCCTCAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TAGGGCTATAATTGTGGCGG | |||||||||||||
| Tm_F_1 | 60.418 | Size_F_1 | 20 | Tm_R_1 | 59.943 | Size_R_1 | 20 | PRODUCT Size | 219 bp | Primer Start | 4688 | Primer End | 4906 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCCACAAAAATCAACACCCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAGGCTAAGTAGGGCGGCTA | |||||||||||||
| Tm_F_2 | 59.836 | Size_F_2 | 20 | Tm_R_2 | 60.364 | Size_R_2 | 20 | PRODUCT Size | 201 bp | Primer Start | 4724 | Primer End | 4924 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACATCTGCAGCCTTCCTCAC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CGGTGATGGGTAGGTTTTGT | |||||||||||||
| Tm_F_3 | 60.418 | Size_F_3 | 20 | Tm_R_3 | 59.711 | Size_R_3 | 20 | PRODUCT Size | 202 bp | Primer Start | 4688 | Primer End | 4889 |