Primers Available
Organism:Acrossocheilus hemispinus mitochondrion
Repeat:(TA)13
Start:16461 End:16486
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CACGAGCCAACAAGTTGAGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGAATATTCAGTGCCGGGTC | |||||||||||||
| Tm_F_1 | 60.025 | Size_F_1 | 20 | Tm_R_1 | 59.009 | Size_R_1 | 20 | PRODUCT Size | 155 bp | Primer Start | 16422 | Primer End | 16576 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTCTTGTCAAACCCCGAAAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGAATATTCAGTGCCGGGTC | |||||||||||||
| Tm_F_2 | 59.948 | Size_F_2 | 20 | Tm_R_2 | 59.009 | Size_R_2 | 20 | PRODUCT Size | 194 bp | Primer Start | 16383 | Primer End | 16576 |
| FORWARD_PRIMER_3 | |||||||||||||
| AAACCCCGAAACCAAGAAAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGAATATTCAGTGCCGGGTC | |||||||||||||
| Tm_F_3 | 60.324 | Size_F_3 | 20 | Tm_R_3 | 59.009 | Size_R_3 | 20 | PRODUCT Size | 186 bp | Primer Start | 16391 | Primer End | 16576 |