Primers Available
Organism:Acrossocheilus hemispinus mitochondrion
Repeat:(CCT)4
Start:10850 End:10861
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGGGAAATCAAACTGAACGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TAGGAATGCGATTAGGCAGC | |||||||||||||
| Tm_F_1 | 59.91 | Size_F_1 | 20 | Tm_R_1 | 60.331 | Size_R_1 | 20 | PRODUCT Size | 206 bp | Primer Start | 10767 | Primer End | 10972 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGGGAAATCAAACTGAACGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTTCTACGTGCGCTTTTGGT | |||||||||||||
| Tm_F_2 | 59.91 | Size_F_2 | 20 | Tm_R_2 | 60.44 | Size_R_2 | 20 | PRODUCT Size | 261 bp | Primer Start | 10767 | Primer End | 11027 |
| FORWARD_PRIMER_3 | |||||||||||||
| TAGCATTTGGCGCTACAGAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGCCTCAGGTGTTGAGTTGT | |||||||||||||
| Tm_F_3 | 59.608 | Size_F_3 | 20 | Tm_R_3 | 60.159 | Size_R_3 | 20 | PRODUCT Size | 252 bp | Primer Start | 10683 | Primer End | 10934 |