Primers Available
Organism:Spinibarbus caldwelli mitochondrion
Repeat:(TTA)4
Start:10762 End:10773
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTAGCCAGCCAAAACCACAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCGTTCGGTTTGATTTCCTC | |||||||||||||
| Tm_F_1 | 60.132 | Size_F_1 | 20 | Tm_R_1 | 60.051 | Size_R_1 | 20 | PRODUCT Size | 195 bp | Primer Start | 10604 | Primer End | 10798 |
| FORWARD_PRIMER_2 | |||||||||||||
| CTAGCCAGCCAAAACCACAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGTTCCTGCATTGAGTCGTT | |||||||||||||
| Tm_F_2 | 60.132 | Size_F_2 | 20 | Tm_R_2 | 60.119 | Size_R_2 | 20 | PRODUCT Size | 210 bp | Primer Start | 10604 | Primer End | 10813 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTGGCCACAGACCCATTATC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCGTTCGGTTTGATTTCCTC | |||||||||||||
| Tm_F_3 | 60.34 | Size_F_3 | 20 | Tm_R_3 | 60.051 | Size_R_3 | 20 | PRODUCT Size | 261 bp | Primer Start | 10538 | Primer End | 10798 |