Primers Available
Organism:Spinibarbus caldwelli mitochondrion
Repeat:(AT)10g(TA)7
Start:16414 End:16448
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAACCCCCAAAACCAAAAAG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGTAGGGGTTTTTAGGCTGC | |||||||||||||
| Tm_F_1 | 60.062 | Size_F_1 | 20 | Tm_R_1 | 59.966 | Size_R_1 | 20 | PRODUCT Size | 143 bp | Primer Start | 16352 | Primer End | 16494 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCAAGAATGTGCAAGCCAAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGTAGGGGTTTTTAGGCTGC | |||||||||||||
| Tm_F_2 | 59.847 | Size_F_2 | 20 | Tm_R_2 | 59.966 | Size_R_2 | 20 | PRODUCT Size | 120 bp | Primer Start | 16375 | Primer End | 16494 |
| FORWARD_PRIMER_3 | |||||||||||||
| CACGCAATCTTGCTACCCTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGTAGGGGTTTTTAGGCTGC | |||||||||||||
| Tm_F_3 | 60.27 | Size_F_3 | 20 | Tm_R_3 | 59.966 | Size_R_3 | 20 | PRODUCT Size | 167 bp | Primer Start | 16328 | Primer End | 16494 |