Primers Available
Organism:Spinibarbus caldwelli mitochondrion
Repeat:(AAATA)3
Start:1139 End:1153
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAGGGGAGGCAAGTCGTAAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCGGTACTGCCTCTATTGCT | |||||||||||||
| Tm_F_1 | 60.496 | Size_F_1 | 20 | Tm_R_1 | 59.504 | Size_R_1 | 20 | PRODUCT Size | 269 bp | Primer Start | 969 | Primer End | 1237 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGAGGCAAGTCGTAACATGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCGGTACTGCCTCTATTGCT | |||||||||||||
| Tm_F_2 | 60.517 | Size_F_2 | 20 | Tm_R_2 | 59.504 | Size_R_2 | 20 | PRODUCT Size | 265 bp | Primer Start | 973 | Primer End | 1237 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTTGGATTAAACCCAGGACG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCGGTACTGCCTCTATTGCT | |||||||||||||
| Tm_F_3 | 59.424 | Size_F_3 | 20 | Tm_R_3 | 59.504 | Size_R_3 | 20 | PRODUCT Size | 225 bp | Primer Start | 1013 | Primer End | 1237 |