Primers Available
Organism:Variola albimarginata mitochondrion
Repeat:(CCTG)3
Start:3507 End:3518
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACCTTTAGCGTTGCTCAGGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GAATAGGGCAAAGGGTCCTC | |||||||||||||
| Tm_F_1 | 60.015 | Size_F_1 | 20 | Tm_R_1 | 59.903 | Size_R_1 | 20 | PRODUCT Size | 180 bp | Primer Start | 3471 | Primer End | 3650 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCAAACCTTTAGCGTTGCTC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAATAGGGCAAAGGGTCCTC | |||||||||||||
| Tm_F_2 | 60.025 | Size_F_2 | 20 | Tm_R_2 | 59.903 | Size_R_2 | 20 | PRODUCT Size | 184 bp | Primer Start | 3467 | Primer End | 3650 |
| FORWARD_PRIMER_3 | |||||||||||||
| CATCTTCACGGGAGGGTTTA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GAATAGGGCAAAGGGTCCTC | |||||||||||||
| Tm_F_3 | 59.926 | Size_F_3 | 20 | Tm_R_3 | 59.903 | Size_R_3 | 20 | PRODUCT Size | 208 bp | Primer Start | 3443 | Primer End | 3650 |