Primers Available
Organism:Lygodium japonicum chloroplast
Repeat:(TTTC)3
Start:63544 End:63555
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAGTTCAGACCCACGTCACC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GATGCAACTCGAGCAATGAA | |||||||||||||
| Tm_F_1 | 60.009 | Size_F_1 | 20 | Tm_R_1 | 59.955 | Size_R_1 | 20 | PRODUCT Size | 136 bp | Primer Start | 63501 | Primer End | 63636 |
| FORWARD_PRIMER_2 | |||||||||||||
| GTTCATCACGCCAACCTTTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GATGCAACTCGAGCAATGAA | |||||||||||||
| Tm_F_2 | 59.978 | Size_F_2 | 20 | Tm_R_2 | 59.955 | Size_R_2 | 20 | PRODUCT Size | 280 bp | Primer Start | 63357 | Primer End | 63636 |
| FORWARD_PRIMER_3 | |||||||||||||
| AAGTTCAGACCCACGTCACC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCAAACGTATTGGGATGCAA | |||||||||||||
| Tm_F_3 | 60.009 | Size_F_3 | 20 | Tm_R_3 | 59.93 | Size_R_3 | 20 | PRODUCT Size | 149 bp | Primer Start | 63501 | Primer End | 63649 |