Primers Available
Organism:Lygodium japonicum chloroplast
Repeat:(GTTG)3gcgtgtttcgaacagataaaatgattggttcagaatattcctaaccgcggtgatttttcaaatatccccgaaatcagat(TTA)4
Start:67985 End:68087
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTCCTTTAGGCCGCTTATGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCCACTGCTTGTCCAGCTA | |||||||||||||
| Tm_F_1 | 59.81 | Size_F_1 | 20 | Tm_R_1 | 59.992 | Size_R_1 | 19 | PRODUCT Size | 278 bp | Primer Start | 67842 | Primer End | 68119 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCTCAATTGGCTGTCTTGCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCCACTGCTTGTCCAGCTA | |||||||||||||
| Tm_F_2 | 60.397 | Size_F_2 | 20 | Tm_R_2 | 59.992 | Size_R_2 | 19 | PRODUCT Size | 169 bp | Primer Start | 67951 | Primer End | 68119 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGAGCAGGGGATTTTGTTCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCCACTGCTTGTCCAGCTA | |||||||||||||
| Tm_F_3 | 60.443 | Size_F_3 | 20 | Tm_R_3 | 59.992 | Size_R_3 | 19 | PRODUCT Size | 191 bp | Primer Start | 67929 | Primer End | 68119 |