Primers Available
Organism:Lygodium japonicum chloroplast
Repeat:(G)13taggccaaaccatgacgggatcaactacgaccgggatcttccgcacca(C)13
Start:132331 End:132404
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGGAAAGGGAGGAAGAAAAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATAGCAATCCCTTTCGCCTT | |||||||||||||
| Tm_F_1 | 60.525 | Size_F_1 | 20 | Tm_R_1 | 60.061 | Size_R_1 | 20 | PRODUCT Size | 171 bp | Primer Start | 132278 | Primer End | 132448 |
| FORWARD_PRIMER_2 | |||||||||||||
| CTCGGAAAGGGAGGAAGAAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATAGCAATCCCTTTCGCCTT | |||||||||||||
| Tm_F_2 | 60.674 | Size_F_2 | 20 | Tm_R_2 | 60.061 | Size_R_2 | 20 | PRODUCT Size | 173 bp | Primer Start | 132276 | Primer End | 132448 |
| FORWARD_PRIMER_3 | |||||||||||||
| TACCATGCTTCTCGGAAAGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GATAGCAATCCCTTTCGCCT | |||||||||||||
| Tm_F_3 | 60.206 | Size_F_3 | 20 | Tm_R_3 | 60.551 | Size_R_3 | 20 | PRODUCT Size | 184 bp | Primer Start | 132266 | Primer End | 132449 |