Primers Available
Organism:Lygodium japonicum chloroplast
Repeat:(A)10
Start:74220 End:74229
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCCAGCAGCATTTTTGACTG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CTCGAGACGCTCTTCAAACC | |||||||||||||
| Tm_F_1 | 59.988 | Size_F_1 | 20 | Tm_R_1 | 60.134 | Size_R_1 | 20 | PRODUCT Size | 271 bp | Primer Start | 74010 | Primer End | 74280 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCCGTGGAACGAAAACTTTA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTCGAGACGCTCTTCAAACC | |||||||||||||
| Tm_F_2 | 59.968 | Size_F_2 | 20 | Tm_R_2 | 60.134 | Size_R_2 | 20 | PRODUCT Size | 146 bp | Primer Start | 74135 | Primer End | 74280 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTCTGTAGAAAACGGGGTGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CTCGAGACGCTCTTCAAACC | |||||||||||||
| Tm_F_3 | 59.964 | Size_F_3 | 20 | Tm_R_3 | 60.134 | Size_R_3 | 20 | PRODUCT Size | 174 bp | Primer Start | 74107 | Primer End | 74280 |