Primers Available
Organism:Medicago truncatula cultivar Borung plastid
Repeat:(AATA)3
Start:5234 End:5245
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGCTTCAACCTTGTCAACCG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGTAACCGCAATTGGAATGA | |||||||||||||
| Tm_F_1 | 60.291 | Size_F_1 | 20 | Tm_R_1 | 59.93 | Size_R_1 | 20 | PRODUCT Size | 217 bp | Primer Start | 5058 | Primer End | 5274 |
| FORWARD_PRIMER_2 | |||||||||||||
| AATTCTCTCGGCCCAGAATC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGTAACCGCAATTGGAATGA | |||||||||||||
| Tm_F_2 | 60.546 | Size_F_2 | 20 | Tm_R_2 | 59.93 | Size_R_2 | 20 | PRODUCT Size | 126 bp | Primer Start | 5149 | Primer End | 5274 |
| FORWARD_PRIMER_3 | |||||||||||||
| AATTCTCTCGGCCCAGAATC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGTTTTGTTGCCGAATTGAT | |||||||||||||
| Tm_F_3 | 60.546 | Size_F_3 | 20 | Tm_R_3 | 59.807 | Size_R_3 | 20 | PRODUCT Size | 209 bp | Primer Start | 5149 | Primer End | 5357 |