Primers Available
Organism:Onychostoma simum mitochondrion
Repeat:(T)11
Start:16152 End:16162
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CATGCCGAGCGTTCTTTTAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAAACCAAAAGAGGGGCACT | |||||||||||||
| Tm_F_1 | 60.23 | Size_F_1 | 20 | Tm_R_1 | 59.976 | Size_R_1 | 20 | PRODUCT Size | 234 bp | Primer Start | 16119 | Primer End | 16352 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGGGTCGCACAATATGAACT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGAGCCTGCACTCTGAAATG | |||||||||||||
| Tm_F_2 | 59.82 | Size_F_2 | 20 | Tm_R_2 | 60.136 | Size_R_2 | 20 | PRODUCT Size | 206 bp | Primer Start | 15992 | Primer End | 16197 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATCAGGGACAAAAATCGTGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGAGCCTGCACTCTGAAATG | |||||||||||||
| Tm_F_3 | 59.79 | Size_F_3 | 20 | Tm_R_3 | 60.136 | Size_R_3 | 20 | PRODUCT Size | 225 bp | Primer Start | 15973 | Primer End | 16197 |