Primers Available
Organism:Onychostoma simum mitochondrion
Repeat:(ATAA)3
Start:1143 End:1154
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGAGAAGACATCCATGCAAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGGTGTGCTGGCTAAATCAT | |||||||||||||
| Tm_F_1 | 59.799 | Size_F_1 | 20 | Tm_R_1 | 59.962 | Size_R_1 | 20 | PRODUCT Size | 273 bp | Primer Start | 1069 | Primer End | 1341 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGAGAAGACATCCATGCAAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTTGGGTGTGCTGGCTAAAT | |||||||||||||
| Tm_F_2 | 59.799 | Size_F_2 | 20 | Tm_R_2 | 60.132 | Size_R_2 | 20 | PRODUCT Size | 276 bp | Primer Start | 1069 | Primer End | 1344 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGGTGCACTTGGATCAAACC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCTCTTTCAGCCTTCCCTTG | |||||||||||||
| Tm_F_3 | 59.973 | Size_F_3 | 20 | Tm_R_3 | 59.545 | Size_R_3 | 20 | PRODUCT Size | 252 bp | Primer Start | 1011 | Primer End | 1262 |