Primers Available
Organism:Najas flexilis chloroplast
Repeat:(AT)6
Start:70454 End:70465
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTGCGCTACATCCCTTTTTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGAACAGTAAATTAGCTTGTCGTG | |||||||||||||
| Tm_F_1 | 59.845 | Size_F_1 | 20 | Tm_R_1 | 59.287 | Size_R_1 | 24 | PRODUCT Size | 253 bp | Primer Start | 70280 | Primer End | 70532 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAAACAAACGCGCTACCAAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGAACAGTAAATTAGCTTGTCGTG | |||||||||||||
| Tm_F_2 | 60.298 | Size_F_2 | 20 | Tm_R_2 | 59.287 | Size_R_2 | 24 | PRODUCT Size | 273 bp | Primer Start | 70260 | Primer End | 70532 |
| FORWARD_PRIMER_3 | |||||||||||||
| AAAACAAACGCGCTACCAAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTAGCTTGTCGTGTCCTTAAAAA | |||||||||||||
| Tm_F_3 | 60.298 | Size_F_3 | 20 | Tm_R_3 | 58.2 | Size_R_3 | 23 | PRODUCT Size | 262 bp | Primer Start | 70260 | Primer End | 70521 |