Primers Available
Organism:Epinephelus sexfasciatus mitochondrion
Repeat:(CCTA)3
Start:13546 End:13557
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTGCCCTAGAACTTGCATCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TAGGCCTCGTTGGGTGTTAC | |||||||||||||
| Tm_F_1 | 60.214 | Size_F_1 | 20 | Tm_R_1 | 59.993 | Size_R_1 | 20 | PRODUCT Size | 257 bp | Primer Start | 13497 | Primer End | 13753 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTGCCCTAGAACTTGCATCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTAGGCCTCGTTGGGTGTTA | |||||||||||||
| Tm_F_2 | 60.214 | Size_F_2 | 20 | Tm_R_2 | 60.125 | Size_R_2 | 20 | PRODUCT Size | 258 bp | Primer Start | 13497 | Primer End | 13754 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTGCCCTAGAACTTGCATCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTACTAGGCCTCGTTGGGTG | |||||||||||||
| Tm_F_3 | 60.214 | Size_F_3 | 20 | Tm_R_3 | 60.125 | Size_R_3 | 20 | PRODUCT Size | 261 bp | Primer Start | 13497 | Primer End | 13757 |