Primers Available
Organism:Secale cereale chloroplast
Repeat:(TTCA)3
Start:64285 End:64296
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACCTACGACATCGGGTTTTG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTTGGAAAAGGGATGTAGCG | |||||||||||||
| Tm_F_1 | 59.853 | Size_F_1 | 20 | Tm_R_1 | 60.067 | Size_R_1 | 20 | PRODUCT Size | 276 bp | Primer Start | 64228 | Primer End | 64503 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTGAACCTACGACATCGGGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTTGGAAAAGGGATGTAGCG | |||||||||||||
| Tm_F_2 | 60.375 | Size_F_2 | 20 | Tm_R_2 | 60.067 | Size_R_2 | 20 | PRODUCT Size | 280 bp | Primer Start | 64224 | Primer End | 64503 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGAACCTACGACATCGGGTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTTGGAAAAGGGATGTAGCG | |||||||||||||
| Tm_F_3 | 60.375 | Size_F_3 | 20 | Tm_R_3 | 60.067 | Size_R_3 | 20 | PRODUCT Size | 279 bp | Primer Start | 64225 | Primer End | 64503 |