Primers Available
Organism:Secale cereale chloroplast
Repeat:(TTC)4
Start:65368 End:65379
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGGCCTCAGAAATCAGAGCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGCCATGAACCTCCTTTTCT | |||||||||||||
| Tm_F_1 | 60.096 | Size_F_1 | 20 | Tm_R_1 | 60.443 | Size_R_1 | 20 | PRODUCT Size | 267 bp | Primer Start | 65191 | Primer End | 65457 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGCCTCAGAAATCAGAGCAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGCCATGAACCTCCTTTTCT | |||||||||||||
| Tm_F_2 | 59.39 | Size_F_2 | 20 | Tm_R_2 | 60.443 | Size_R_2 | 20 | PRODUCT Size | 266 bp | Primer Start | 65192 | Primer End | 65457 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGGCCTCAGAAATCAGAGCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CTTGGCCATGAACCTCCTT | |||||||||||||
| Tm_F_3 | 60.096 | Size_F_3 | 20 | Tm_R_3 | 60.058 | Size_R_3 | 19 | PRODUCT Size | 270 bp | Primer Start | 65191 | Primer End | 65460 |