Primers Available
Organism:Secale cereale chloroplast
Repeat:(TTATA)3
Start:100509 End:100523
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGACTACTGGCGGTTTCATT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCGAAAATCGCTATTCCACC | |||||||||||||
| Tm_F_1 | 60.132 | Size_F_1 | 20 | Tm_R_1 | 60.038 | Size_R_1 | 20 | PRODUCT Size | 219 bp | Primer Start | 100396 | Primer End | 100614 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGACTACTGGCGGTTTCATT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CAGATCCGTTTTTCGAGTCC | |||||||||||||
| Tm_F_2 | 60.132 | Size_F_2 | 20 | Tm_R_2 | 59.67 | Size_R_2 | 20 | PRODUCT Size | 151 bp | Primer Start | 100396 | Primer End | 100546 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTCATTATGGGGCAGCTCAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCGAAAATCGCTATTCCACC | |||||||||||||
| Tm_F_3 | 60.435 | Size_F_3 | 20 | Tm_R_3 | 60.038 | Size_R_3 | 20 | PRODUCT Size | 205 bp | Primer Start | 100410 | Primer End | 100614 |