Primers Available
Organism:Secale cereale chloroplast
Repeat:(TCCT)3acagctgatagggaaaaatcgttgtttttacgatccctatgtagaaagccctttttttctagtatttactagaaaatttgatcctctc(T)11
Start:42915 End:43025
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGACCCAGTCGCTCACTAAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TATGGCCGTGATCTGTCATT | |||||||||||||
| Tm_F_1 | 60.277 | Size_F_1 | 20 | Tm_R_1 | 58.953 | Size_R_1 | 20 | PRODUCT Size | 275 bp | Primer Start | 42797 | Primer End | 43071 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGACCCAGTCGCTCACTAAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AATATGGCCGTGATCTGTCA | |||||||||||||
| Tm_F_2 | 60.277 | Size_F_2 | 20 | Tm_R_2 | 58.953 | Size_R_2 | 20 | PRODUCT Size | 277 bp | Primer Start | 42797 | Primer End | 43073 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGTTGTATCGACCCAGTCGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGCCGTGATCTGTCATTACG | |||||||||||||
| Tm_F_3 | 60.142 | Size_F_3 | 20 | Tm_R_3 | 61.468 | Size_R_3 | 20 | PRODUCT Size | 280 bp | Primer Start | 42789 | Primer End | 43068 |