Primers Available
Organism:Secale cereale chloroplast
Repeat:(T)14
Start:36182 End:36195
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGCGTCCAATAGGATTTGAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACCGGCTCCATACTCCTTCT | |||||||||||||
| Tm_F_1 | 59.112 | Size_F_1 | 20 | Tm_R_1 | 60.096 | Size_R_1 | 20 | PRODUCT Size | 231 bp | Primer Start | 36085 | Primer End | 36315 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGCGTCCAATAGGATTTGAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAGGCTCATAACCTTGGGGT | |||||||||||||
| Tm_F_2 | 59.112 | Size_F_2 | 20 | Tm_R_2 | 59.827 | Size_R_2 | 20 | PRODUCT Size | 270 bp | Primer Start | 36085 | Primer End | 36354 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGCGTCCAATAGGATTTGAAC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACCGGCTCCATACTCCTTCT | |||||||||||||
| Tm_F_3 | 59.952 | Size_F_3 | 21 | Tm_R_3 | 60.096 | Size_R_3 | 20 | PRODUCT Size | 231 bp | Primer Start | 36085 | Primer End | 36315 |