Primers Available
Organism:Secale cereale chloroplast
Repeat:(T)13
Start:7223 End:7235
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCAGCCTTTCTACTCCCCTG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCTTACCACACGGATCCACT | |||||||||||||
| Tm_F_1 | 59.425 | Size_F_1 | 20 | Tm_R_1 | 59.844 | Size_R_1 | 20 | PRODUCT Size | 194 bp | Primer Start | 7092 | Primer End | 7285 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCAGCCTTTCTACTCCCCTG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACACGGATCCACTAGGATGG | |||||||||||||
| Tm_F_2 | 59.425 | Size_F_2 | 20 | Tm_R_2 | 59.803 | Size_R_2 | 20 | PRODUCT Size | 187 bp | Primer Start | 7092 | Primer End | 7278 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCAGCCTTTCTACTCCCCTG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CACGGATCCACTAGGATGGT | |||||||||||||
| Tm_F_3 | 59.425 | Size_F_3 | 20 | Tm_R_3 | 59.803 | Size_R_3 | 20 | PRODUCT Size | 186 bp | Primer Start | 7092 | Primer End | 7277 |