Primers Available
Organism:Secale cereale chloroplast
Repeat:(T)12
Start:47229 End:47240
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGACATAGCAAACGATTAATCACA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCTCTTTGTCCTCGTCCGAT | |||||||||||||
| Tm_F_1 | 59.559 | Size_F_1 | 24 | Tm_R_1 | 59.803 | Size_R_1 | 20 | PRODUCT Size | 247 bp | Primer Start | 47104 | Primer End | 47350 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCAAACGATTAATCACAGAACTCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCTCTTTGTCCTCGTCCGAT | |||||||||||||
| Tm_F_2 | 60.527 | Size_F_2 | 24 | Tm_R_2 | 59.803 | Size_R_2 | 20 | PRODUCT Size | 240 bp | Primer Start | 47111 | Primer End | 47350 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGACATAGCAAACGATTAATCACA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCTCTCTTTGTCCTCGTCCG | |||||||||||||
| Tm_F_3 | 59.559 | Size_F_3 | 24 | Tm_R_3 | 60.523 | Size_R_3 | 20 | PRODUCT Size | 249 bp | Primer Start | 47104 | Primer End | 47352 |