Primers Available
Organism:Secale cereale chloroplast
Repeat:(T)11
Start:8273 End:8283
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CAACTCCTTTGCGCTACACA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTCCCTCTCTATTGCATGGG | |||||||||||||
| Tm_F_1 | 60.05 | Size_F_1 | 20 | Tm_R_1 | 60.029 | Size_R_1 | 20 | PRODUCT Size | 100 bp | Primer Start | 8229 | Primer End | 8328 |
| FORWARD_PRIMER_2 | |||||||||||||
| CAACTCCTTTGCGCTACACA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCTTCTTTTCCCATTCCCTC | |||||||||||||
| Tm_F_2 | 60.05 | Size_F_2 | 20 | Tm_R_2 | 59.875 | Size_R_2 | 20 | PRODUCT Size | 113 bp | Primer Start | 8229 | Primer End | 8341 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGACTTAGACCGCGCAAGAC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTCCCTCTCTATTGCATGGG | |||||||||||||
| Tm_F_3 | 59.644 | Size_F_3 | 20 | Tm_R_3 | 60.029 | Size_R_3 | 20 | PRODUCT Size | 189 bp | Primer Start | 8140 | Primer End | 8328 |