Primers Available
Organism:Secale cereale chloroplast
Repeat:(T)10
Start:79893 End:79902
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGCCTTTCGGAATGAAACTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAATCGATACGATCCATGTCA | |||||||||||||
| Tm_F_1 | 60.192 | Size_F_1 | 20 | Tm_R_1 | 57.9 | Size_R_1 | 21 | PRODUCT Size | 224 bp | Primer Start | 79727 | Primer End | 79950 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTGCCTTTCGGAATGAAACT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAATCGATACGATCCATGTCA | |||||||||||||
| Tm_F_2 | 59.685 | Size_F_2 | 20 | Tm_R_2 | 57.9 | Size_R_2 | 21 | PRODUCT Size | 225 bp | Primer Start | 79726 | Primer End | 79950 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTTTCATTGCCTTTCGGAAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AAATCGATACGATCCATGTCA | |||||||||||||
| Tm_F_3 | 59.525 | Size_F_3 | 20 | Tm_R_3 | 57.9 | Size_R_3 | 21 | PRODUCT Size | 231 bp | Primer Start | 79720 | Primer End | 79950 |