Primers Available
Organism:Secale cereale chloroplast
Repeat:(T)10
Start:17770 End:17779
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGCCATGGATCAATCAGTTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGTATATTCGGGCGTCCTTT | |||||||||||||
| Tm_F_1 | 60.289 | Size_F_1 | 20 | Tm_R_1 | 60.171 | Size_R_1 | 20 | PRODUCT Size | 219 bp | Primer Start | 17584 | Primer End | 17802 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCCATGGATCAATCAGTTCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGTATATTCGGGCGTCCTTT | |||||||||||||
| Tm_F_2 | 59.456 | Size_F_2 | 20 | Tm_R_2 | 60.171 | Size_R_2 | 20 | PRODUCT Size | 218 bp | Primer Start | 17585 | Primer End | 17802 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGGCCATGGATCAATCAGTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGTATATTCGGGCGTCCTTT | |||||||||||||
| Tm_F_3 | 59.366 | Size_F_3 | 20 | Tm_R_3 | 60.171 | Size_R_3 | 20 | PRODUCT Size | 220 bp | Primer Start | 17583 | Primer End | 17802 |