Primers Available
Organism:Secale cereale chloroplast
Repeat:(GTT)4
Start:31547 End:31558
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TAGCTTTCGACTTTTCGGGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATAGGCTGCGGCTAATGTTG | |||||||||||||
| Tm_F_1 | 59.953 | Size_F_1 | 20 | Tm_R_1 | 60.251 | Size_R_1 | 20 | PRODUCT Size | 157 bp | Primer Start | 31506 | Primer End | 31662 |
| FORWARD_PRIMER_2 | |||||||||||||
| TAGCTTTCGACTTTTCGGGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATGACCCTCCATGGATTCAC | |||||||||||||
| Tm_F_2 | 59.953 | Size_F_2 | 20 | Tm_R_2 | 59.593 | Size_R_2 | 20 | PRODUCT Size | 181 bp | Primer Start | 31506 | Primer End | 31686 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGGAATATATTGGCGGATGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATAGGCTGCGGCTAATGTTG | |||||||||||||
| Tm_F_3 | 59.581 | Size_F_3 | 20 | Tm_R_3 | 60.251 | Size_R_3 | 20 | PRODUCT Size | 141 bp | Primer Start | 31522 | Primer End | 31662 |