Primers Available
Organism:Secale cereale chloroplast
Repeat:(ATA)4
Start:56468 End:56479
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCATGAGACTTGACAAATCGAGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTGACTCACGATTACGCCTG | |||||||||||||
| Tm_F_1 | 59.867 | Size_F_1 | 23 | Tm_R_1 | 59.864 | Size_R_1 | 20 | PRODUCT Size | 240 bp | Primer Start | 56405 | Primer End | 56644 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCATGAGACTTGACAAATCGAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTGACTCACGATTACGCCTG | |||||||||||||
| Tm_F_2 | 57.951 | Size_F_2 | 22 | Tm_R_2 | 59.864 | Size_R_2 | 20 | PRODUCT Size | 240 bp | Primer Start | 56405 | Primer End | 56644 |
| FORWARD_PRIMER_3 | |||||||||||||
| CATGAGACTTGACAAATCGAGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTGACTCACGATTACGCCTG | |||||||||||||
| Tm_F_3 | 57.951 | Size_F_3 | 22 | Tm_R_3 | 59.864 | Size_R_3 | 20 | PRODUCT Size | 239 bp | Primer Start | 56406 | Primer End | 56644 |