Primers Available
Organism:Secale cereale chloroplast
Repeat:(AGAA)3
Start:68618 End:68629
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTCCAGGAATCCGGTAAGGT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGCATTCGAATCACCCATTC | |||||||||||||
| Tm_F_1 | 60.319 | Size_F_1 | 20 | Tm_R_1 | 59.9 | Size_R_1 | 20 | PRODUCT Size | 280 bp | Primer Start | 68507 | Primer End | 68786 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCCAGGAATCCGGTAAGGTA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGCATTCGAATCACCCATTC | |||||||||||||
| Tm_F_2 | 59.382 | Size_F_2 | 20 | Tm_R_2 | 59.9 | Size_R_2 | 20 | PRODUCT Size | 279 bp | Primer Start | 68508 | Primer End | 68786 |
| FORWARD_PRIMER_3 | |||||||||||||
| CATCTCCAGGAATCCGGTAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCGAATCACCCATTCCTTTT | |||||||||||||
| Tm_F_3 | 59.887 | Size_F_3 | 20 | Tm_R_3 | 59.363 | Size_R_3 | 20 | PRODUCT Size | 278 bp | Primer Start | 68504 | Primer End | 68781 |