Primers Available
Organism:Secale cereale chloroplast
Repeat:(AAT)5
Start:24652 End:24666
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACCCAAGACATGCTTATCGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CATCGTAGCCACAAGGGACT | |||||||||||||
| Tm_F_1 | 59.955 | Size_F_1 | 20 | Tm_R_1 | 60.134 | Size_R_1 | 20 | PRODUCT Size | 233 bp | Primer Start | 24540 | Primer End | 24772 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACCCAAGACATGCTTATCGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GTTGATCCAGGTTCCATCGT | |||||||||||||
| Tm_F_2 | 59.955 | Size_F_2 | 20 | Tm_R_2 | 59.786 | Size_R_2 | 20 | PRODUCT Size | 247 bp | Primer Start | 24540 | Primer End | 24786 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCGCTATTGGGGATCCTATT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CATCGTAGCCACAAGGGACT | |||||||||||||
| Tm_F_3 | 60.13 | Size_F_3 | 20 | Tm_R_3 | 60.134 | Size_R_3 | 20 | PRODUCT Size | 262 bp | Primer Start | 24511 | Primer End | 24772 |