Primers Available
Organism:Secale cereale chloroplast
Repeat:(AACG)3
Start:98882 End:98893
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTCCCTAGAGCCTCCGGTAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATTTGATCAGGCCGTGTAGG | |||||||||||||
| Tm_F_1 | 60.053 | Size_F_1 | 20 | Tm_R_1 | 59.955 | Size_R_1 | 20 | PRODUCT Size | 208 bp | Primer Start | 98719 | Primer End | 98926 |
| FORWARD_PRIMER_2 | |||||||||||||
| CTCTCTCCTCCGACTTCCCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATTTGATCAGGCCGTGTAGG | |||||||||||||
| Tm_F_2 | 59.945 | Size_F_2 | 20 | Tm_R_2 | 59.955 | Size_R_2 | 20 | PRODUCT Size | 222 bp | Primer Start | 98705 | Primer End | 98926 |
| FORWARD_PRIMER_3 | |||||||||||||
| GATGAGTGCTCTCTCCTCCG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATTTGATCAGGCCGTGTAGG | |||||||||||||
| Tm_F_3 | 60.096 | Size_F_3 | 20 | Tm_R_3 | 59.955 | Size_R_3 | 20 | PRODUCT Size | 230 bp | Primer Start | 98697 | Primer End | 98926 |