Primers Available
Organism:Secale cereale chloroplast
Repeat:(A)13
Start:104608 End:104620
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGGGTTTAGTTGGTTAAATTCAAAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGGAACAAGAGAGCTGTTTCAT | |||||||||||||
| Tm_F_1 | 60.03 | Size_F_1 | 25 | Tm_R_1 | 58.983 | Size_R_1 | 22 | PRODUCT Size | 173 bp | Primer Start | 104525 | Primer End | 104697 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTTTTCCAAAATTTATCTCATTGAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCATTGGAATTCACTCAACCAG | |||||||||||||
| Tm_F_2 | 58.254 | Size_F_2 | 25 | Tm_R_2 | 59.971 | Size_R_2 | 22 | PRODUCT Size | 253 bp | Primer Start | 104474 | Primer End | 104726 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTTTTCCAAAATTTATCTCATTGAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCATTGGAATTCACTCAACCA | |||||||||||||
| Tm_F_3 | 58.254 | Size_F_3 | 25 | Tm_R_3 | 58.961 | Size_R_3 | 21 | PRODUCT Size | 253 bp | Primer Start | 104474 | Primer End | 104726 |