Primers Available
Organism:Secale cereale chloroplast
Repeat:(A)12
Start:7949 End:7960
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTTTAGCGGGCATTTCCATA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCAGAATAGCGGATAGAGGCA | |||||||||||||
| Tm_F_1 | 60.054 | Size_F_1 | 20 | Tm_R_1 | 59.952 | Size_R_1 | 21 | PRODUCT Size | 222 bp | Primer Start | 7865 | Primer End | 8086 |
| FORWARD_PRIMER_2 | |||||||||||||
| AATCGATATTGGCCGAGAGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCAGAATAGCGGATAGAGGCA | |||||||||||||
| Tm_F_2 | 59.628 | Size_F_2 | 20 | Tm_R_2 | 59.952 | Size_R_2 | 21 | PRODUCT Size | 260 bp | Primer Start | 7827 | Primer End | 8086 |
| FORWARD_PRIMER_3 | |||||||||||||
| GATTTTCTCTTTAGCGGGCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCAGAATAGCGGATAGAGGCA | |||||||||||||
| Tm_F_3 | 59.433 | Size_F_3 | 20 | Tm_R_3 | 59.952 | Size_R_3 | 21 | PRODUCT Size | 230 bp | Primer Start | 7857 | Primer End | 8086 |