Primers Available
Organism:Secale cereale chloroplast
Repeat:(A)12
Start:29538 End:29549
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCTACGAGGTCGCATTGATT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAATCTCTCATTTCCGACGC | |||||||||||||
| Tm_F_1 | 60.096 | Size_F_1 | 20 | Tm_R_1 | 59.273 | Size_R_1 | 20 | PRODUCT Size | 171 bp | Primer Start | 29405 | Primer End | 29575 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTGGTACCGGATTCCAAAAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAATCTCTCATTTCCGACGC | |||||||||||||
| Tm_F_2 | 60.16 | Size_F_2 | 20 | Tm_R_2 | 59.273 | Size_R_2 | 20 | PRODUCT Size | 103 bp | Primer Start | 29473 | Primer End | 29575 |
| FORWARD_PRIMER_3 | |||||||||||||
| TATCGGGCAATCTTATTGGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AAATCTCTCATTTCCGACGC | |||||||||||||
| Tm_F_3 | 59.751 | Size_F_3 | 20 | Tm_R_3 | 59.273 | Size_R_3 | 20 | PRODUCT Size | 275 bp | Primer Start | 29301 | Primer End | 29575 |