Primers Available
Organism:Secale cereale chloroplast
Repeat:(A)11
Start:33564 End:33574
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGGGAAATAGCTAATGGCAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CATGGTCAGCAAAGTTGTTTCT | |||||||||||||
| Tm_F_1 | 60.054 | Size_F_1 | 20 | Tm_R_1 | 59.286 | Size_R_1 | 22 | PRODUCT Size | 280 bp | Primer Start | 33403 | Primer End | 33682 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGGGAAATAGCTAATGGCAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CATGGTCAGCAAAGTTGTTTC | |||||||||||||
| Tm_F_2 | 60.054 | Size_F_2 | 20 | Tm_R_2 | 58.269 | Size_R_2 | 21 | PRODUCT Size | 280 bp | Primer Start | 33403 | Primer End | 33682 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCGAATCGACGACCTATGTA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AAGAGGACTCTTCCGCCTAGA | |||||||||||||
| Tm_F_3 | 58.599 | Size_F_3 | 20 | Tm_R_3 | 59.607 | Size_R_3 | 21 | PRODUCT Size | 205 bp | Primer Start | 33514 | Primer End | 33718 |