Primers Available
Organism:Secale cereale chloroplast
Repeat:(A)11
Start:2594 End:2604
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCGAGAAAAACCAGGGTACA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCATCTTTGCATTTATTGCG | |||||||||||||
| Tm_F_1 | 59.964 | Size_F_1 | 20 | Tm_R_1 | 59.169 | Size_R_1 | 20 | PRODUCT Size | 276 bp | Primer Start | 2404 | Primer End | 2679 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTCCGAGAAAAACCAGGGTA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCATCTTTGCATTTATTGCG | |||||||||||||
| Tm_F_2 | 59.541 | Size_F_2 | 20 | Tm_R_2 | 59.169 | Size_R_2 | 20 | PRODUCT Size | 278 bp | Primer Start | 2402 | Primer End | 2679 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCCAAAATGTTCCATCTTCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCATCTTTGCATTTATTGCG | |||||||||||||
| Tm_F_3 | 59.22 | Size_F_3 | 20 | Tm_R_3 | 59.169 | Size_R_3 | 20 | PRODUCT Size | 252 bp | Primer Start | 2428 | Primer End | 2679 |