Primers Available
Organism:Secale cereale chloroplast
Repeat:(A)10
Start:47890 End:47899
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCTCGTGTCACCAGTTCAAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCGTCGGAATAGCGGAATAC | |||||||||||||
| Tm_F_1 | 59.72 | Size_F_1 | 20 | Tm_R_1 | 60.06 | Size_R_1 | 20 | PRODUCT Size | 276 bp | Primer Start | 47852 | Primer End | 48127 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCCTCGTGTCACCAGTTCAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCGTCGGAATAGCGGAATAC | |||||||||||||
| Tm_F_2 | 60.285 | Size_F_2 | 20 | Tm_R_2 | 60.06 | Size_R_2 | 20 | PRODUCT Size | 277 bp | Primer Start | 47851 | Primer End | 48127 |
| FORWARD_PRIMER_3 | |||||||||||||
| GTTCAAATCTGGTTCCTGGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCGTCGGAATAGCGGAATAC | |||||||||||||
| Tm_F_3 | 59.532 | Size_F_3 | 20 | Tm_R_3 | 60.06 | Size_R_3 | 20 | PRODUCT Size | 263 bp | Primer Start | 47865 | Primer End | 48127 |