Primers Available
Organism:Secale cereale chloroplast
Repeat:(A)10
Start:3391 End:3400
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCCTGGAAAGAAAGGGAGTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAGGGATGGGATTGAATGTG | |||||||||||||
| Tm_F_1 | 60.045 | Size_F_1 | 20 | Tm_R_1 | 59.605 | Size_R_1 | 20 | PRODUCT Size | 194 bp | Primer Start | 3312 | Primer End | 3505 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCCTGGAAAGAAAGGGAGTC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGGATTGAATAAGGGATGGG | |||||||||||||
| Tm_F_2 | 60.045 | Size_F_2 | 20 | Tm_R_2 | 59.576 | Size_R_2 | 20 | PRODUCT Size | 204 bp | Primer Start | 3312 | Primer End | 3515 |
| FORWARD_PRIMER_3 | |||||||||||||
| AAACCCTGGAAAGAAAGGGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AAGGGATGGGATTGAATGTG | |||||||||||||
| Tm_F_3 | 59.912 | Size_F_3 | 20 | Tm_R_3 | 59.605 | Size_R_3 | 20 | PRODUCT Size | 197 bp | Primer Start | 3309 | Primer End | 3505 |