Primers Available
Organism:Secale cereale chloroplast
Repeat:(A)10
Start:33836 End:33845
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GAAACAACTTTGCTGACCATGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTCCCGAACCAAACATGAAT | |||||||||||||
| Tm_F_1 | 60.154 | Size_F_1 | 22 | Tm_R_1 | 60.17 | Size_R_1 | 20 | PRODUCT Size | 232 bp | Primer Start | 33662 | Primer End | 33893 |
| FORWARD_PRIMER_2 | |||||||||||||
| GAAACAACTTTGCTGACCATGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCCGAACCAAACATGAATCT | |||||||||||||
| Tm_F_2 | 60.154 | Size_F_2 | 22 | Tm_R_2 | 59.79 | Size_R_2 | 20 | PRODUCT Size | 230 bp | Primer Start | 33662 | Primer End | 33891 |
| FORWARD_PRIMER_3 | |||||||||||||
| GAAACAACTTTGCTGACCATGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATCTCTTCCCGAACCAAACA | |||||||||||||
| Tm_F_3 | 60.154 | Size_F_3 | 22 | Tm_R_3 | 59.526 | Size_R_3 | 20 | PRODUCT Size | 237 bp | Primer Start | 33662 | Primer End | 33898 |