Primers Available
Organism:Secale cereale chloroplast
Repeat:(A)10
Start:32512 End:32521
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGTTGTGGCACTAGCGCTTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGGGGTATTATCTGTCCCGA | |||||||||||||
| Tm_F_1 | 60.081 | Size_F_1 | 20 | Tm_R_1 | 60.148 | Size_R_1 | 20 | PRODUCT Size | 203 bp | Primer Start | 32464 | Primer End | 32666 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGTTGTGGCACTAGCGCTTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCCCTTCCTAGAATGGGGTA | |||||||||||||
| Tm_F_2 | 60.081 | Size_F_2 | 20 | Tm_R_2 | 60.278 | Size_R_2 | 20 | PRODUCT Size | 216 bp | Primer Start | 32464 | Primer End | 32679 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTGTGGCACTAGCGCTTTTA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGGGGTATTATCTGTCCCGA | |||||||||||||
| Tm_F_3 | 59.648 | Size_F_3 | 20 | Tm_R_3 | 60.148 | Size_R_3 | 20 | PRODUCT Size | 201 bp | Primer Start | 32466 | Primer End | 32666 |