Primers Available
Organism:Pachygrapsus crassipes mitochondrion
Repeat:(TCTT)4
Start:14454 End:14469
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TAATGGGCTCATACCCCGTA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCCAATTCAAGCTCCAAATC | |||||||||||||
| Tm_F_1 | 60.167 | Size_F_1 | 20 | Tm_R_1 | 59.651 | Size_R_1 | 20 | PRODUCT Size | 147 bp | Primer Start | 14394 | Primer End | 14540 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGCTCATACCCCGTAAATGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCCAATTCAAGCTCCAAATC | |||||||||||||
| Tm_F_2 | 59.784 | Size_F_2 | 20 | Tm_R_2 | 59.651 | Size_R_2 | 20 | PRODUCT Size | 142 bp | Primer Start | 14399 | Primer End | 14540 |
| FORWARD_PRIMER_3 | |||||||||||||
| TAATGGGCTCATACCCCGTA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGGAGGGTGGAACCTAAAGC | |||||||||||||
| Tm_F_3 | 60.167 | Size_F_3 | 20 | Tm_R_3 | 59.576 | Size_R_3 | 20 | PRODUCT Size | 260 bp | Primer Start | 14394 | Primer End | 14653 |