Primers Available
Organism:Onychostoma barbatulum mitochondrion
Repeat:(TA)13
Start:16471 End:16496
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTCTTGTCAAACCCCGAAAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AATTTTTGGCAGGGGTTTTC | |||||||||||||
| Tm_F_1 | 59.948 | Size_F_1 | 20 | Tm_R_1 | 60.164 | Size_R_1 | 20 | PRODUCT Size | 162 bp | Primer Start | 16392 | Primer End | 16553 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCGAAACCAAAAAGGACTCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AATTTTTGGCAGGGGTTTTC | |||||||||||||
| Tm_F_2 | 60.081 | Size_F_2 | 20 | Tm_R_2 | 60.164 | Size_R_2 | 20 | PRODUCT Size | 149 bp | Primer Start | 16405 | Primer End | 16553 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCCCTACGCCCTAATAATCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AATTTTTGGCAGGGGTTTTC | |||||||||||||
| Tm_F_3 | 59.659 | Size_F_3 | 20 | Tm_R_3 | 60.164 | Size_R_3 | 20 | PRODUCT Size | 187 bp | Primer Start | 16367 | Primer End | 16553 |