Primers Available
Organism:Ascaridia columbae mitochondrion
Repeat:(ATTT)3
Start:12860 End:12871
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCCATTGTTGTTTTTAGTGGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCAACTCTCCCAAAAACATATCC | |||||||||||||
| Tm_F_1 | 59.37 | Size_F_1 | 21 | Tm_R_1 | 59.363 | Size_R_1 | 23 | PRODUCT Size | 161 bp | Primer Start | 12754 | Primer End | 12914 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGCCATTGTTGTTTTTAGTGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTCTCCCAAAAACATATCCACA | |||||||||||||
| Tm_F_2 | 58.594 | Size_F_2 | 21 | Tm_R_2 | 58.949 | Size_R_2 | 22 | PRODUCT Size | 158 bp | Primer Start | 12753 | Primer End | 12910 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGCCATTGTTGTTTTTAGTGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCTCCCAAAAACATATCCACA | |||||||||||||
| Tm_F_3 | 58.594 | Size_F_3 | 21 | Tm_R_3 | 57.93 | Size_R_3 | 21 | PRODUCT Size | 157 bp | Primer Start | 12753 | Primer End | 12909 |