Primers Available
Organism:Epinephelus trimaculatus mitochondrion
Repeat:(TTC)4
Start:14775 End:14786
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATGCTCATATCGGACGAGGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AATCCGCCTCAGATTCATTG | |||||||||||||
| Tm_F_1 | 60.591 | Size_F_1 | 20 | Tm_R_1 | 60.036 | Size_R_1 | 20 | PRODUCT Size | 220 bp | Primer Start | 14705 | Primer End | 14924 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCCTCACAGGCCTCTTCCTA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGTCCTCAGGGAAGGACGTA | |||||||||||||
| Tm_F_2 | 59.943 | Size_F_2 | 20 | Tm_R_2 | 60.646 | Size_R_2 | 20 | PRODUCT Size | 277 bp | Primer Start | 14555 | Primer End | 14831 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGGCTGACTAATCCGAAACA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AATCCGCCTCAGATTCATTG | |||||||||||||
| Tm_F_3 | 59.272 | Size_F_3 | 20 | Tm_R_3 | 60.036 | Size_R_3 | 20 | PRODUCT Size | 279 bp | Primer Start | 14646 | Primer End | 14924 |