Primers Available
Organism:Epinephelus trimaculatus mitochondrion
Repeat:(CCA)4
Start:4282 End:4293
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTCATAGCCCAACAGCATCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGGATGGCAATAGTGGTTGC | |||||||||||||
| Tm_F_1 | 59.823 | Size_F_1 | 20 | Tm_R_1 | 59.962 | Size_R_1 | 20 | PRODUCT Size | 173 bp | Primer Start | 4190 | Primer End | 4362 |
| FORWARD_PRIMER_2 | |||||||||||||
| CTCATAGCCCAACAGCATCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CGAGGATGGCAATAGTGGTT | |||||||||||||
| Tm_F_2 | 59.823 | Size_F_2 | 20 | Tm_R_2 | 59.955 | Size_R_2 | 20 | PRODUCT Size | 175 bp | Primer Start | 4190 | Primer End | 4364 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACCCTTGCCATCCTACCTCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GAGGACTTCGGGTAGTCACG | |||||||||||||
| Tm_F_3 | 59.957 | Size_F_3 | 20 | Tm_R_3 | 59.721 | Size_R_3 | 20 | PRODUCT Size | 249 bp | Primer Start | 4172 | Primer End | 4420 |