Primers Available
Organism:Halocynthia spinosa complete mitochondrial genome
Repeat:(TTG)4
Start:9975 End:9986
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTGGTTCGTTGGTTTCATCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCTCACGAACAACATCTCGC | |||||||||||||
| Tm_F_1 | 59.941 | Size_F_1 | 20 | Tm_R_1 | 59.992 | Size_R_1 | 20 | PRODUCT Size | 103 bp | Primer Start | 9926 | Primer End | 10028 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTGGTTCGTTGGTTTCATCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGAACCATCCCAATACGCAG | |||||||||||||
| Tm_F_2 | 59.941 | Size_F_2 | 20 | Tm_R_2 | 59.955 | Size_R_2 | 20 | PRODUCT Size | 164 bp | Primer Start | 9926 | Primer End | 10089 |
| FORWARD_PRIMER_3 | |||||||||||||
| GTTCTTTTGCGTAAGGCGAC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCTCACGAACAACATCTCGC | |||||||||||||
| Tm_F_3 | 59.889 | Size_F_3 | 20 | Tm_R_3 | 59.992 | Size_R_3 | 20 | PRODUCT Size | 196 bp | Primer Start | 9833 | Primer End | 10028 |