Primers Available
Organism:Halocynthia spinosa complete mitochondrial genome
Repeat:(T)17
Start:4474 End:4490
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGCGTTTTTGGTTTTGTTGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CAAAAGGGCAAAACTACCCA | |||||||||||||
| Tm_F_1 | 60.011 | Size_F_1 | 20 | Tm_R_1 | 59.968 | Size_R_1 | 20 | PRODUCT Size | 222 bp | Primer Start | 4323 | Primer End | 4544 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCTTGGTGTTGATGGTAGCG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CAAAAGGGCAAAACTACCCA | |||||||||||||
| Tm_F_2 | 59.716 | Size_F_2 | 20 | Tm_R_2 | 59.968 | Size_R_2 | 20 | PRODUCT Size | 238 bp | Primer Start | 4307 | Primer End | 4544 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTTTGGTTTTGTTGGAGCGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACCAACAACCCAGAAACTGC | |||||||||||||
| Tm_F_3 | 60.514 | Size_F_3 | 20 | Tm_R_3 | 60.012 | Size_R_3 | 20 | PRODUCT Size | 280 bp | Primer Start | 4328 | Primer End | 4607 |