Primers Available
Organism:Halocynthia spinosa complete mitochondrial genome
Repeat:(T)11
Start:8473 End:8483
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGAGCAAATCAACACGGTTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACCTCCCGATAAGATCCACC | |||||||||||||
| Tm_F_1 | 59.978 | Size_F_1 | 20 | Tm_R_1 | 60.154 | Size_R_1 | 20 | PRODUCT Size | 260 bp | Primer Start | 8439 | Primer End | 8698 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGAGCAAATCAACACGGTTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTCCCGATAAGATCCACCCT | |||||||||||||
| Tm_F_2 | 59.978 | Size_F_2 | 20 | Tm_R_2 | 60.285 | Size_R_2 | 20 | PRODUCT Size | 258 bp | Primer Start | 8439 | Primer End | 8696 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCGTGTTGTCTTGTCCACAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACCTCCCGATAAGATCCACC | |||||||||||||
| Tm_F_3 | 60.194 | Size_F_3 | 20 | Tm_R_3 | 60.154 | Size_R_3 | 20 | PRODUCT Size | 279 bp | Primer Start | 8420 | Primer End | 8698 |